Fig. 2

Analysis of mitochondrial superoxide production. SKBR3, MDA-MB-231, MCF-7 and B16 F10 cells were treated with either MitoPQ (positive control) or VAE for 24 h, labelled with MitoSOX red Superoxide Indicator and analyzed by flow cytometry. A Representative FACS images of MitoSOX red fluorescence intensity and B Box and whiskers plots represent the MFI data (Min to Max) from at least 3 independent experiments. Significance values are given relative to untreated controls (*p < 0.05, **p < 0.01, ***p < 0.001)