Fig. 1

The expression levels of p-eIF2α, ATF4 and CHOP after treatment with indicated reagents for 24 h were determined by stain free Western blot analysis. Normalized data are presented as mean ± SE of at least 3 independent experiments with SKBR3, MDA-MB-231, MCF-7 and B16F10 cells. Representative western blot images are shown for all cell lines. The arrow indicates the location of ATF4 protein bands. Significance values are given relative to untreated controls (*p < 0.05, **p < 0.01, ***p < 0.001)