Fig. 9
Effects of compound D, compound D acetate, DMPBD, and PE on H2O2 -induced apoptosis in C2C12 myoblast cells. Panels show the percentage of cell apoptosis following treatment with (A) compound D, (B) compound D acetate, (C) DMPBD, (D) PE, (E) western blot analysis of Bax, Bcl2, cleaved caspase-3, and procaspase-3 protein bands in response to all phenylbutenoids and PE, and (F) Relative expression of Bax/Bcl2 and cleaved caspase-3/procaspase-3 protein levels. All data are presented as the mean ± SEM from four independent experiments (n = 4). Different lowercase letters indicate significant differences between samples at p < 0.05, as determined by ANOVA followed by LSD post-hoc test. CD: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-ol (compound D), DA: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-yl acetate (compound D acetate), DMPBD: (E)-(3,4-dimethoxyphenyl)butadiene, PE: phenylbutenoid extract, NAC: N-acetylcysteine