Fig. 8

Inhibition of ROS production in C2C12 myoblast cells treated with H₂O₂ and various compounds, as measured by the DCFH₂-DA assay. Panels show ROS production following treatment with (A) compound D, (B) compound D acetate, (C) DMPBD, and (D) PE. All data are expressed as the mean ± SEM from four independent experiments (n = 4). Different lowercase letters indicate significant differences between samples at p < 0.05, as determined by ANOVA followed by LSD post-hoc test. CD: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-ol (compound D), DA: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-yl acetate (compound D acetate), DMPBD: (E)-(3,4-dimethoxyphenyl)butadiene, PE: phenylbutenoid extract, NAC: N-acetylcysteine