Fig. 7

Suppression of Akt, ERK, and p38MAPK signaling pathways by compound D, compound D acetate, DMPBD, and PE in LPS-induced inflammation in RAW264.7 macrophages. (A) Western blot analysis of ERK, p38 MAPK, and Akt protein bands in response to all phenylbutenoids and PE treatment, (B) Relative expression of p-ERK/ERK protein, (C) Relative expression of p-p38/p38 protein, and (D) Relative expression of p-Akt/Akt protein. All values are presented as the mean ± SEM from four independent experiments (n = 4). Different lowercase letters indicate significant differences between samples at p < 0.05, as determined by ANOVA followed by LSD post-hoc test. CD: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-ol (compound D), DA: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-yl acetate (compound D acetate), DMPBD: (E)-(3,4-dimethoxyphenyl)butadiene, PE: phenylbutenoid extract, Dex: dexamethasone, LPS: lipopolysaccharide