Fig. 6

Effect of compound D, compound D acetate, DMPBD, and PE on p65 NF-κB translocation in LPS-inflamed RAW264.7 macrophages. Immunofluorescence staining for NF-κB p65 was observed under fluorescence microscopy after treatment with all tested compounds in the presence and absence of LPS. Nuclei were visualized using Hoechst 33342. CD: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-ol (compound D), DA: (E)-1-(3,4-dimethoxyphenyl)but-3-en-1-yl acetate (compound D acetate), DMPBD: (E)-(3,4-dimethoxyphenyl)butadiene, PE: phenylbutenoid extract, Dex: dexamethasone, LPS: lipopolysaccharide