Fig. 6
Apoptosis induction activity after 48 h of exposure to IC10, IC20, IC30 and IC50 concentrations of F-EtOAc and F10 on (A) EoL-1 and (B) MV4-11 cells. The percentages represent the proportion of total events within each respective categories, as gated in the flow cytometry plots. The bar graphs represent Annexin V + cells (%) which are in early and late apoptosis as determined by Annexin V/PI staining, analyzed by flow cytometry after incubation with F-EtOAc and F10 on (C) EoL-1 and (D) MV4-11 cells. Doxorubicin was used as positive control (PC) in this experiment. The percentage of apoptotic cells in each treatment was statistically compared with vehicle control (VC). The bars represent the mean ± SD of three independent experiments. The asterisk (*) indicates significant difference compared to VC; (*p < 0.05, **p < 0.001, one-way ANOVA)