Fig. 4
Cell cycle distribution of (A) EoL-1 and (B) MV4-11 cells after incubation with IC10, IC15, and IC20 concentrations of F-EtOAc and F10 for 48 h. Cell distribution along cell-cycle phases was investigated by flow cytometry analysis of the DNA content after PI staining. Each bar graph shows the cell cycle phases of (C) EoL-1 and (D) MV4-11 cells after incubation with F-EtOAc and F10 for 48 h in comparison with vehicle control (VC). The bars represent the mean ± SD of three independent experiments. The asterisk (*) indicates significant difference compared to VC; (*p < 0.05, **p < 0.001, one-way ANOVA)