Fig. 4
From: Anti-central fatigue effects of myelophil in 5-HTergic hyperactivity mice model

Effects of Myelophil (MYP) on 5-HTergic activity-related molecules in the raphe nuclei (RN). Relative 5-HT levels in the DRN were measured using immunofluorescence (A) and semi-quantified (B). Western blot analysis was used to assess levels of 5-HTT, TPH2, VMAT2, MAO-A, and 5-HT1AR protein expressions in the RN (C), with semi-quantification (D). The data are expressed as the means and standard deviations (n = 3). #, p < 0.05 and ##, p < 0.01 compared with the normal group; *, p < 0.05 and **, p < 0.01 compared with the Flx group