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Table 1 PCR primers for amplification of PKS and lipopeptide genes

From: Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening

Target gene

Primers

Sequences (5’- 3’)

Amplicon size (bp)

KS domain

KS-F

KS-R

GCGATGGATCCNCAGCAGCG

GTGCCGGTNCCGTGNGYYTC

680

sfp

sfp-F

sfp-R

ATGAAGATTTACGGAATTTA

TTATAAAAGCTCTTCGTACG

675

ItuD

ItuD1-F

ItuD1-R

GATGCGATCTCCTTGGATGT

ATCGTCATGTGCTGCTTGAG

647