Fig. 2

PCR amplification detects the presence of some genes related to the biosynthesis of bioactive metabolites in the rhizosphere strain, Bacillus velezensis DM, on a 1% agarose gel electrophoresis using a gradient of eight annealing temperatures to verify the initial PCR results as follows: Polyketide synthase which encodes KS domain (A) and lipopeptide genes for surfactin and iturin A, including 4’-phosphopantetheinyl transferase (B) and malonyl-CoA transacylase (C), respectively