Fig. 8
Western blot analysis following curcumin, shogaol, and nocodazole treatments in KG-1a cells. KG-1a cells were incubated with IC50 concentration of curcumin, shogaol, and nocodazole (positive control) for 0–12 h. The expression of target proteins was analyzed by Western blotting. (A) Protein levels after treatment with the IC50 concentrations of curcumin (10 µg/mL), shogaol (3 µg/mL), and nocodazole (10 ng/mL) for 0–12 h. (B) Protein expressions for cleaved caspase-3, (C) cleaved PARP, (D) WT1, and (E) TAZ in KG-1a cells after 0–12 h of treatment. The levels of protein were normalized using GAPDH protein levels. Full-length blots are presented in Supplementary file (uncropped gels and blot images, Figs. S17–S21). Shogaol induced the activation of cleaved caspase-3 and PARP at 3 h, while curcumin triggered these proteins at 6 h. WT1 expression significantly decreased with both curcumin and shogaol treatments, but nocodazole had no effect. TAZ levels remained unchanged. Data are the mean ± SD (n = 3); *p < 0.05 vs. VC