Fig. 4
Effect of crude ethanolic extracts and their active compounds treatments on CD34 and WT1 protein expressions in KG-1a cells. KG-1a cells were treated with crude ethanolic extracts and their active compounds with IC20 concentrations for 48 h. CD34 and WT1 protein levels were determined by Western blotting. (A) Representative images and (B) representative bar graph of inhibitory effects of crude ethanolic extracts and their active compounds on WT1 and CD34 protein expressions in KG-1a cells for 48 h. The levels of protein were normalized using GAPDH protein levels. Full-length blots are presented in Supplementary file (Figs. S14–S16). All compounds and extracts inhibited WT1 and CD34 expressions significantly by more than 20% compared to VC. Data are the mean ± SD (n = 3); *p < 0.05 vs. VC